The \emph{fractional matching preclusion number} of a graph $G$, denoted by $fmp(G)$, is the minimum number of edges whose deletion results in a graph that has no fractional perfect matchings. In this paper, we first give some sharp upper and lower bounds of fractional matching preclusion number. Next, graphs with large and small fractional matching preclusion number are characterized, respectively. In the end, we investigate some extremal problems on fractional matching preclusion number.