A conference matrix of order $n$ is an $n\times n$ matrix $C$ with diagonal entries $0$ and off-diagonal entries $\pm 1$ satisfying $CC^\top=(n-1)I$. If $C$ is symmetric, then $C$ has a symmetric spectrum $Σ$ (that is, $Σ=-Σ$) and eigenvalues $\pm\sqrt{n-1}$. We show that many principal submatrices of $C$ also have symmetric spectrum, which leads to examples of Seidel matrices of graphs (or, equivalently, adjacency matrices of complete signed graphs) with a symmetric spectrum. In addition, we show that some Seidel matrices with symmetric spectrum can be characterized by this construction.