The study and calculation of spectrum of networks can be used to describe networks structure and quantify analysis of networks performance. The fractal Möbius octagonal networks, denoted by $Q_n$, is derived from the inverse identification of the opposite lateral edges of fractal linear octagonal networks. In this paper, the normalized Laplacian spectrum of $Q_n$ is determined by two matrices $\mathcal {L}_A$ and $\mathcal {L}_S$. As an important application of our results, some topological indices (multiplicative degree-Kirchhoff index, the number of spanning trees) formulas of $Q_n$ are obtained.