We consider configurations of lines in 3-space with incidences prescribed by a graph. This defines a subvariety in a product of Grassmannians. Leveraging a connection with rigidity theory in the plane, for any graph, we determine the dimension of the incidence variety and characterize when it is irreducible or a complete intersection. We study its multidegree and the family of Schubert problems it encodes. Our spanning-tree coordinates enable efficient symbolic computations. We also provide numerical irreducible decompositions for incidence varieties with up to eight lines. These constructions with lines play a key role in the Landau analysis of scattering amplitudes in particle physics.