The detection of the abnormal area from urban data is a significant research problem. However, to the best of our knowledge, previous methods designed on spatio-temporal anomalies are road-based or grid-based, which usually causes the data sparsity problem and affects the detection results. In this paper, we proposed a dynamic region partition method to address the above issues. Besides, we proposed an unsupervised REgional Anomaly Detection framework (ReAD) to detect abnormal regions with arbitrary shapes by jointly considering spatial and temporal properties. Specifically, the proposed framework first generate regions via a dynamic region partition method. It keeps that observations in the same region have adjacent locations and similar non-spatial attribute readings, and could alleviate data sparsity and heterogeneity compared with the grid-based approach. Then, an anomaly metric will be calculated for each region by a regional divergence calculation method. The abnormal regions could be finally detected by a weighted approach or a wavy approach according to the different scenario. Experiments on both the simulated dataset and real-world applications demonstrate the effectiveness and practicability of the proposed framework.