The rapid advancement in large language models (LLMs) has demonstrated significant potential in End-to-End Software Development (E2ESD). However, existing E2ESD benchmarks are limited by coarse-grained requirement specifications and unreliable evaluation protocols, hindering a true understanding of current framework capabilities. To address these limitations, we present E2EDev, a novel benchmark grounded in the principles of Behavior-Driven Development (BDD), which evaluates the capabilities of E2ESD frameworks by assessing whether the generated software meets user needs through mimicking real user interactions (Figure 1). E2EDev comprises (i) a fine-grained set of user requirements, (ii) multiple BDD test scenarios with corresponding Python step implementations for each requirement, and (iii) a fully automated testing pipeline built on the Behave framework. To ensure its quality while reducing the annotation effort, E2EDev leverages our proposed Human-in-the-Loop Multi-Agent Annotation Framework (HITL-MAA). By evaluating various E2ESD frameworks and LLM backbones with E2EDev, our analysis reveals a persistent struggle to effectively solve these tasks, underscoring the critical need for more effective and cost-efficient E2ESD solutions. Our codebase and benchmark are publicly available at https://github.com/SCUNLP/E2EDev.