We study the problem of separating audio sources from a single linear mixture. The goal is to find a decomposition of the single channel spectrogram into a sum of individual contributions associated to a certain number of sources. In this paper, we consider an informed source separation problem in which the input spectrogram is partly annotated. We propose a convex formulation that relies on a nuclear norm penalty to induce low rank for the contributions. We show experimentally that solving this model with a simple subgradient method outperforms a previously introduced nonnegative matrix factorization (NMF) technique, both in terms of source separation quality and computation time.