The search for extraterrestrial life has long been a primary focus of scientific exploration, driven by rapid advancements in technology and our understanding of the universe. The discovery of water on Mars has sparked significant interest, raising the question of whether life could exist on the planet. This study proposes a novel approach to simulate and illustrate the detection of life using a proof-of-life module integrated into a Mars rover. The module is an autonomous system capable of traveling to designated regions, excavating soil, collecting samples, and performing biochemical testing onboard the rover itself. The project is inherently multidisciplinary, integrating mechanical systems such as a drill mechanism and a vacuum system, alongside biochemical analysis for soil testing. The module is capable of successfully detecting the presence or absence of living components of life from the collected soil particles. This proof-of-life module serves as a proof-of-concept for autonomous life detection in extraterrestrial environments and lays the foundation for future exploration missions.