We consider subsequences with gap constraints, i.e., length-k subsequences p that can be embedded into a string w such that the induced gaps (i.e., the factors of w between the positions to which p is mapped to) satisfy given gap constraints $gc = (C_1, C_2, ..., C_{k-1})$; we call p a gc-subsequence of w. In the case where the gap constraints gc are defined by lower and upper length bounds $C_i = (L^-_i, L^+_i) \in \mathbb{N}^2$ and/or regular languages $C_i \in REG$, we prove tight (conditional on the orthogonal vectors (OV) hypothesis) complexity bounds for checking whether a given p is a gc-subsequence of a string w. We also consider the whole set of all gc-subsequences of a string, and investigate the complexity of the universality, equivalence and containment problems for these sets of gc-subsequences.