Denial of service (DoS) attacks and more particularly the distributed ones (DDoS) are one of the latest threat and pose a grave danger to users, organizations and infrastructures of the Internet. Several schemes have been proposed on how to detect some of these attacks, but they suffer from a range of problems, some of them being impractical and others not being effective against these attacks. This paper reports the design principles and evaluation results of our proposed framework that autonomously detects and accurately characterizes a wide range of flooding DDoS attacks in ISP network. Attacks are detected by the constant monitoring of propagation of abrupt traffic changes inside ISP network. For this, a newly designed flow-volume based approach (FVBA) is used to construct profile of the traffic normally seen in the network, and identify anomalies whenever traffic goes out of profile. Consideration of varying tolerance factors make proposed detection system scalable to the varying network conditions and attack loads in real time. Six-sigma method is used to identify threshold values accurately for malicious flows characterization. FVBA has been extensively evaluated in a controlled test-bed environment. Detection thresholds and efficiency is justified using receiver operating characteristics (ROC) curve. For validation, KDD 99, a publicly available benchmark dataset is used. The results show that our proposed system gives a drastic improvement in terms of detection and false alarm rate.