In the present era of advanced technology, the Internet of Things (IoT) plays a crucial role in enabling smart connected environments. This includes various domains such as smart homes, smart healthcare, smart cities, smart vehicles, and many others.With ubiquitous smart connected devices and systems, a large amount of data associated with them is at a prime risk from malicious entities (e.g., users, devices, applications) in these systems. Innovative technologies, including cloud computing, Machine Learning (ML), and data analytics, support the development of anomaly detection models for the Vehicular Internet of Things (V-IoT), which encompasses collaborative automatic driving and enhanced transportation systems. However, traditional centralized anomaly detection models fail to provide better services for connected vehicles due to issues such as high latency, privacy leakage, performance overhead, and model drift. Recently, Federated Learning (FL) has gained significant recognition for its ability to address data privacy concerns in the IoT domain. Digital Twin (DT), proves beneficial in addressing uncertain crises and data security issues by creating a virtual replica that simulates various factors, including traffic trajectories, city policies, and vehicle utilization. However, the effectiveness of a V-IoT DT system heavily relies on the collection of long-term and high-quality data to make appropriate decisions. This paper introduces a Hierarchical Federated Learning (HFL) based anomaly detection model for V-IoT, aiming to enhance the accuracy of the model. Our proposed model integrates both DT and HFL approaches to create a comprehensive system for detecting malicious activities using an anomaly detection model. Additionally, real-world V-IoT use case scenarios are presented to demonstrate the application of the proposed model.