One of the most significant threats faced by enterprise networks today is from Bots. A Bot is a program that operates as an agent for a user and runs automated tasks over the internet, at a much higher rate than would be possible for a human alone. A collection of Bots in a network, used for malicious purposes is referred to as a Botnet. Bot attacks can range from localized attacks like key-logging to network intensive attacks like Distributed Denial of Service (DDoS). In this paper, we suggest a novel approach that can detect and combat Bots. The proposed solution adopts a two pronged strategy which we have classified into the standalone algorithm and the network algorithm. The standalone algorithm runs independently on each node of the network. It monitors the active processes on the node and tries to identify Bot processes using parameters such as response time and output to input traffic ratio. If a suspicious process has been identified the network algorithm is triggered. The network algorithm will then analyze conversations to and from the hosts of the network using the transport layer flow records. It then tries to deduce the Bot pattern as well as Bot signatures which can subsequently be used by the standalone algorithm to thwart Bot processes at their very onset.