About Wine, I think it ships a new WoW64 mode that can run on a 64bit host without even any 32bit libraries or 32bit syscalls.
So your modern 32bit Wine installation is a bunch of 64bit ELFs, and the NT syscalls far jump to 64bit mode with ye old Heaven's Gate.

I believe on ARM this actually lets you run 32bit Windows apps without any 32bit support from the host, since the x86 instructions are JIT-ed anyways.
On x86 I believe (?) the kernel only needs to leave a 32bit descriptor laying around in the GDT, you could maybe remove all other IA32 support and Wine might keep working?
(Of course all of this is still unrelated to x32)