Popular transformer detectors have achieved promising performance through query-based learning using attention mechanisms. However, the roles of existing decoder query types (e.g., content query and positional query) are still underexplored. These queries are generally predefined with a fixed number (fixed-query), which limits their flexibility. We find that the learning of these fixed-query is impaired by Recurrent Opposing inTeractions (ROT) between two attention operations: Self-Attention (query-to-query) and Cross-Attention (query-to-encoder), thereby degrading decoder efficiency. Furthermore, "query ambiguity" arises when shared-weight decoder layers are processed with both one-to-one and one-to-many label assignments during training, violating DETR's one-to-one matching principle. To address these challenges, we propose DS-Det, a more efficient detector capable of detecting a flexible number of objects in images. Specifically, we reformulate and introduce a new unified Single-Query paradigm for decoder modeling, transforming the fixed-query into flexible. Furthermore, we propose a simplified decoder framework through attention disentangled learning: locating boxes with Cross-Attention (one-to-many process), deduplicating predictions with Self-Attention (one-to-one process), addressing "query ambiguity" and "ROT" issues directly, and enhancing decoder efficiency. We further introduce a unified PoCoo loss that leverages box size priors to prioritize query learning on hard samples such as small objects. Extensive experiments across five different backbone models on COCO2017 and WiderPerson datasets demonstrate the general effectiveness and superiority of DS-Det. The source codes are available at https://github.com/Med-Process/DS-Det/.