Locating specific segments within an instructional video is an efficient way to acquire guiding knowledge. Generally, the task of obtaining video segments for both verbal explanations and visual demonstrations is known as visual answer localization (VAL). However, users often need multiple interactions to obtain answers that align with their expectations when using the system. During these interactions, humans deepen their understanding of the video content by asking themselves questions, thereby accurately identifying the location. Therefore, we propose a new task, named In-VAL, to simulate the multiple interactions between humans and videos in the procedure of obtaining visual answers. The In-VAL task requires interactively addressing several semantic gap issues, including 1) the ambiguity of user intent in the input questions, 2) the incompleteness of language in video subtitles, and 3) the fragmentation of content in video segments. To address these issues, we propose Ask2Loc, a framework for resolving In-VAL by asking questions. It includes three key modules: 1) a chatting module to refine initial questions and uncover clear intentions, 2) a rewriting module to generate fluent language and create complete descriptions, and 3) a searching module to broaden local context and provide integrated content. We conduct extensive experiments on three reconstructed In-VAL datasets. Compared to traditional end-to-end and two-stage methods, our proposed Ask2Loc can improve performance by up to 14.91 (mIoU) on the In-VAL task. Our code and datasets can be accessed at https://github.com/changzong/Ask2Loc.