Text-to-video generation has advanced rapidly, but existing methods typically output only the final composited video and lack editable layered representations, limiting their use in professional workflows. We propose \textbf{LayerT2V}, a unified multi-layer video generation framework that produces multiple semantically consistent outputs in a single inference pass: the full video, an independent background layer, and multiple foreground RGB layers with corresponding alpha mattes. Our key insight is that recent video generation backbones use high compression in both time and space, enabling us to serialize multiple layer representations along the temporal dimension and jointly model them on a shared generation trajectory. This turns cross-layer consistency into an intrinsic objective, improving semantic alignment and temporal coherence. To mitigate layer ambiguity and conditional leakage, we augment a shared DiT backbone with LayerAdaLN and layer-aware cross-attention modulation. LayerT2V is trained in three stages: alpha mask VAE adaptation, joint multi-layer learning, and multi-foreground extension. We also introduce \textbf{VidLayer}, the first large-scale dataset for multi-layer video generation. Extensive experiments demonstrate that LayerT2V substantially outperforms prior methods in visual fidelity, temporal consistency, and cross-layer coherence.