Text-to-image (T2I) generation models often struggle with multi-instance synthesis (MIS), where they must accurately depict multiple distinct instances in a single image based on complex prompts detailing individual features. Traditional MIS control methods for UNet architectures like SD v1.5/SDXL fail to adapt to DiT-based models like FLUX and SD v3.5, which rely on integrated attention between image and text tokens rather than text-image cross-attention. To enhance MIS in DiT, we first analyze the mixed attention mechanism in DiT. Our token-wise and layer-wise analysis of attention maps reveals a hierarchical response structure: instance tokens dominate early layers, background tokens in middle layers, and attribute tokens in later layers. Building on this observation, we propose a training-free approach for enhancing MIS in DiT-based models with hierarchical and step-layer-wise attention specialty tuning (AST). AST amplifies key regions while suppressing irrelevant areas in distinct attention maps across layers and steps, guided by the hierarchical structure. This optimizes multimodal interactions by hierarchically decoupling the complex prompts with instance-based sketches. We evaluate our approach using upgraded sketch-based layouts for the T2I-CompBench and customized complex scenes. Both quantitative and qualitative results confirm our method enhances complex layout generation, ensuring precise instance placement and attribute representation in MIS.