Multimodal large language models (MLLMs) have achieved remarkable progress on various visual question answering and reasoning tasks leveraging instruction fine-tuning specific datasets. They can also learn from preference data annotated by human to enhance their reasoning ability and mitigate hallucinations. Most of preference data is generated from the model itself. However, existing methods require high-quality critical labels, which are costly and rely on human or proprietary models like GPT-4V. In this work, we propose Enhancing Alignment in MLLMs via Critical Observation (EACO), which aligns MLLMs by self-generated preference data using only 5k images economically. Our approach begins with collecting and refining a Scoring Evaluation Instruction-tuning dataset to train a critical evaluation model, termed the Critic. This Critic observes model responses across multiple dimensions, selecting preferred and non-preferred outputs for refined Direct Preference Optimization (DPO) tuning. To further enhance model performance, we employ an additional supervised fine-tuning stage after preference tuning. EACO reduces the overall hallucinations by 65.6% on HallusionBench and improves the reasoning ability by 21.8% on MME-Cognition. EACO achieves an 8.5% improvement over LLaVA-v1.6-Mistral-7B across multiple benchmarks. Remarkably, EACO also shows the potential critical ability in open-source MLLMs, demonstrating that EACO is a viable path to boost the competence of MLLMs.