Existing large language models (LLMs) occasionally generate plausible yet factually incorrect responses, known as hallucinations. Two main approaches have been proposed to mitigate hallucinations: retrieval-augmented language models (RALMs) and refusal post-training. However, current research predominantly focuses on their individual effectiveness while overlooking the evaluation of the refusal capability of RALMs. Ideally, if RALMs know when they do not know, they should refuse to answer.In this study, we ask the fundamental question: Do RALMs know when they don't know? Specifically, we investigate three questions. First, are RALMs well calibrated with respect to different internal and external knowledge states? We examine the influence of various factors. Contrary to expectations, when all retrieved documents are irrelevant, RALMs still tend to refuse questions they could have answered correctly. Next, given the model's pronounced \textbf{over-refusal} behavior, we raise a second question: How does a RALM's refusal ability align with its calibration quality? Our results show that the over-refusal problem can be mitigated through in-context fine-tuning. However, we observe that improved refusal behavior does not necessarily imply better calibration or higher overall accuracy. Finally, we ask: Can we combine refusal-aware RALMs with uncertainty-based answer abstention to mitigate over-refusal? We develop a simple yet effective refusal mechanism for refusal-post-trained RALMs that improves their overall answer quality by balancing refusal and correct answers. Our study provides a more comprehensive understanding of the factors influencing RALM behavior. Meanwhile, we emphasize that uncertainty estimation for RALMs remains an open problem deserving deeper investigation.