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var t = new Timer(TimerCallback);

// Figure how much time until 4:00
DateTime now = DateTime.Now;
DateTime fourOClock = DateTime.Today.AddHours(16.0);

// If it's already past 4:00, wait until 4:00 tomorrow    
if (now > fourOClock)
{
    fourOClock = fourOClock.AddDays(1.0);
}

int msUntilFour = (int)((fourOClock - now).TotalMilliseconds);

// Set the timer to elapse only once, at 4:00.
t.Change(msUntilFour, Timeout.Infinite);